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(Ramesh Chand Nagarwal in Singapore)
Mr. Ramesh Chand Nagarwal, Senior Research Scholar, Department of Pharmaceutical Engineering presented his Research Paper on “5-Fluorouracil Loaded Chitosan Coated PLA Nanoparticles for Ophthalmic Drug Delivery: In vitro and In vivo Evaluation in the Rabbit Eye” at ASIA-ARVO 2011 (International Meeting on Research in Vision and Ophthalmology), held from January 20-24, 2011 at the Resorts World Sentosa (RWS), Singapore.
Financial assistance for attending the conference was given by BHU. He has submitted PhD thesis entitled “Studies on Polymeric Nanoparticulate Systems of 5-Fluorouracil for Ophthalmic Delivery” under the supervision of Prof. J. K. Pandit.
Ramesh C. Nagarwal
Senior Research Scholar
Department of Pharmaceutical Engineering,
Institute of Technology,
Banaras Hindu University,
Varanasi-221005
Email id: rcnagarwal.rs.phe@itbhu.ac.in, jkpandit@bhu.ac.in,
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Abstract of the Research Paper:
“5-Fluorouracil Loaded Chitosan Coated PLA Nanoparticles for Ophthalmic Drug Delivery: In vitro and In vivo Evaluation in the Rabbit Eye”
Authors- Ramesh Chand Nagarwal, S Kant, P. N. Singh and J. K. Pandit
Purpose: The objective of the present study was to develop a 5-fluorouracil loaded chitosan coated poly (D, L-lactic acid) nanoparticles (CH-PLA NPs) for ocular drug delivery and Ex in vivo and in-vivo evaluation in the anterior segment of New Zealand rabbits eye.
Method: PLA nanoparticles (PLA-NPs) loaded 5-fluorouracil (5-FU) were prepared by nano-precipitation method using varying concentration and molecular weight of PLA to regulate the particle size. PLA-NPs were coated by overnight of the NPs in chitosan solution and separated by centrifugation and subsequently dried by lyophilization. The dimension and shape of nanoparticles were verified by using dynamic light scattering (DLS), atomic force microscope (AFM) and scanning electron microscope (SEM). In-vitro mucus (porcine stomach, type II) interaction with nanoparticles was assessed by viscosity change measured by Brookfield viscometer. Ex-vivo study was conducted with goat and rabbit cornea using diffusion cell. In-vivo experiment was conducted in New Zealand female rabbit’s eye and 5-FU concentration was measured in aqueous and vitreous humor samples by HPLC. Ocular tolerability of developed nanoparticles was evaluated by modified Draize test.
Results: The drug loading and in-vitro releasing capability was regulated as 6 to 22 % and 35 to 60%, respectively, by synchronizing drug loaded nanoparticle dimension. In-vitro experiments indicated a diffusion controlled release of 5-FU in phosphate buffer solution (pH 7.4). Mucus interaction study with CH-PLA NPs did not show interaction between the mucus solution and the suspension of NPs, indicating that the CH-PLA NPs will be free from irritation of the cornea. In-vitro permeation was higher with rabbit cornea as compared to goat cornea. Uncoated PLA and CH adsorbed PLA-NPs did not show any sign of ocular inflammation or tissue damage in the rabbit eye. PLA NPs and CH coated PLA-NPs showed higher 5-FU level in the aqueous humor compared to the aqueous solution of 5-FU, but the CH-Coated PLA NPs significantly increased the 5-FU level as compared to PLA-NPs.
Conclusion: CH-PLA NPs has a potential for ocular delivery for the prolonged treatment of conjunctiva/corneal squamous cell carcinoma.
About Research Paper:
The ocular efficacy of topically applied drugs is influenced by corneal contact time. A common method to improve drug ocular bioavailability is to increase precorneal residence time using mucoadhesive polymers. Cationic chitosan has attracted considerable attention due to its unique properties such as good biocompatibility, biodegradability and the ability to enhance paracellular transport of drugs. PLA nanoparticles have negative surface charge that is not suitable for ocular delivery. Cornea has a negative surface charge that needed a cationic surface charge having nanoparticles for better retention. In this study we have developed Chitosan coated PLA nanoparticles that have positive surface charge due to coating of chitosan. In-vitro and in-vivo study in rabbit eye supported the possibility of Chitosan coated PLA nanoparticles for ocular delivery with improved 5-FU bioavailability.
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Additional links:
*Asia-ARVO-2011 conference at Singapore (20-22 January, 2011)
http://asiaarvo2011.com.sg/Index/
Asia-ARVO is the premier eye research meeting in Asia, held every two years in different locations. Asia-ARVO showcases major clinical, basic and translational aspects of vision and ophthalmic research, with a particular focus on Asian diseases and conditions. The Asia-ARVO meeting reflects ARVO's commitment to partnering with the many countries in Asia that have expanding eye research programs.
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*Article Published in the Journal of Biomedical nanotechnology
Chitosan Coated PLA Nanoparticles for Ophthalmic Delivery: Characterization,In-Vitro and In-Vivo Study in Rabbit Eye
http://www.ingentaconnect.com/content/asp/jbn/2010/00000006/00000006/art00004?crawler=true
Authors: Nagarwal, Ramesh C.; Singh, P.N.; Kant, S.; Maiti, P.; Pandit, J.K.
Source: Journal of Biomedical Nanotechnology, Volume 6, Number 6, December 2010 , pp. 648-657(10)
Publisher: American Scientific Publishers
Abstract:
The objective of the present study is to develop a poly (D, L-lactic acid) (PLA) nano-carrier for topical ocular applications. PLA nanoparticles (PLA-NPs) with 5-fluorouracil were prepared using varying concentration and molecular weight of PLA to regulate the particle size. The dimension and shape of nanoparticles were verified by using dynamic light scattering (DLS), atomic force microscope (AFM) and scanning electron microscope (SEM). Ex-vivo permeation study was conducted by goat and rabbit excised cornea. In-vivo experiment was conducted in rabbit eye and 5-FU concentration was measured in aqueous and vitreous humor by HPLC. In-vitro experiments indicated a diffusion controlled release of 5-FU. No significant interaction was observed in between mucin and PLA NPs that measured in terms of viscosity change. Ex-vivo permeation was significantly higher with rabbit cornea as compared to goat cornea. PLA and CH-PLA DNPs showed increased level of 5-FU as comparison to 5-FU solution. In-vivo study showed significantly higher concentration in case of uncoated and CH coated PLA nanoparticles in rabbit eye as compared to free 5-FU solution. PLA nanoparticle was found non-irritant in nature by modified Draize test.
Document Type: Research article
DOI: 10.1166/jbn.2010.1168
Publication date: 2010-12-01
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*Department of Pharmaceutical Engineering, IT-BHU, Varanasi, India
List of research Scholars
http://itbhu.ac.in/phe/indexae82.html?option=com_content&view=article&id=11&Itemid=13
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(if you having troubles, try posting your comment on this page or send an email to chronicle @ itbhuglobal.org)Institute of Technology, Banaras Hindu University
Varanasi 221005, UP